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Measuring a second messenger (e.g., cAMP or Ca 2+ ) supports pathway engagement, but proving that the pathway is causally responsible for the cellular response requires additional evidence such as receptor antagonists, pathway inhibitors, receptor knockdown or knockout, or orthogonal rescue experiments

However, despite the low carriage rate, in some Asian countries, the mortality rate of IMD is high due to weak surveillance and/or the lack of routine vaccination (Wang et al., 2019)